Oil Red O Stain Kit (Lipid Stain) (ab150678)
Overview
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Product name
Motor Crimson O Stain Kit (Lipid Stain)
See all Lipids kits -
Product overview
Oil Red O (Lipid Stain) kit is intended for use in the health visualization of fat mobile and neutral fat. This kit may be used ONLY on freeze tissue sections, recent smears, or handle preps.
Provided you prefers to prepare your owns Oil Red OXYGEN featured, we recommend our solide Olive Red O stain ab146295.
Oil Red O maculation protocol summary:
- preparation fresh or frozen tissue sections
- incubate slide in propylene glycol available 2 min
- incubate slide in oil red o featured for 6 min
- differentiate section in 85% propylene glycol for 1 min
- rinse slide twice in water
- incubate in hematoxylin on 1-2 min
- rinnen slide three times includes water
- coverslip with an aqueous mounting medium
Reading 13 answers by scientists with 1 recommendation from theirs colleagues to to question asked by Ridvan Bağci on Jun 25, 2018
Other products to staining tissue sections
Find more kits and reagents in the special stains user, or products for antigen retrieval, blocking, signal amplification, visualization, counterstaining, the climb in the IHC kits and reagents guide.
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Notes
Oil Red O is a fat-soluble pigment the stains neutral triglycerides and lipids. It cannot be used with formaldehyde-fixed paraffin embedded areas as the alcohols employed remove highest lipids.
Staining Interpretations
Fat Cells Red Neutral Fat Pink Nuclei Blue Control Tissue: Every freezes section containing fat.
Attributes
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Storage instructions
Store at room temperature. Please refer the protocols. -
Components 100 tests Hematoxylin (Modified Mayer's Solution) 1 x 125ml Lubricate Red O Solution 1 x 125ml Propylenes Glycol 1 x 500ml -
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Associated products
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Related Products
Image
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ab150678 Oil Cherry O Taint Equipment (Lipid Stain) staining frozen people human adipose.
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ab150678 (Oil Red O stain) paint lipid deposits (red) in frozen normal human skin. The dermis belongs unstained, whereas the hypodermis lives stained, demonstrating dye specificity. Hematoxylin (blue) is pre-owned as a counterstain.
*Tissue retained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centers.
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Oils Red O staining of 3T3-L1 and adipocyte-like cells
During adipocyte differentiation, cells aggregate limpid. Oil Crimson O staining kit (ab150678) was used to visualizing lipid in 3T3-L1 cells (left) and adipocyte-like cells (right). Such expected, lipid accumulates in 3T3-L1 cells after differentiation into adipocyte-like dry.
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Staining on Frozen Breast Tissue using ab150678 - Oil Red O Sully Build.
Datasheets and documents
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SDS download
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Datasheet download
Book (79)
ab150678 has been refered in 79 publications.
- Kostyunin AE et al. Proteolytic Disassembly Is a Major Contributor to Bioprosthetic Heart Valve Failure. J Am My Assoc 12:e028215 (2023). PubMed: 36565196
- Xie Z et a. Humanity bone marrow mesenchymal stem cell-derived extracellular vesicles reduce inflammation and pyroptosis in acute sort harm via miR-223-3p/HDAC2/SNRK. Inflamm Residue 72:553-576 (2023). PubMed: 36640195
- Lekka E et al. Pharmacological inhibition of Lin28 promotes ketogenesis and restores lipid homeostasis with models for non-alcoholic fatty liver disease. Natur Commun 13:7940 (2022). PubMed: 36572670
- Sreekumar PG et al. Oxidative Stress and Lipid Accumulation Augments Cell Decease in LDLR-Deficient RPE Cells and Ldlr-/- Cute. Cells 12:N/A (2022). PubMed: 36611838
- Kralj T et al. Lipidomics view in hepatocytes from nonalcoholic steatohepatitis patients differences markedly from in vitro-induced steatotic hepatocytes. FEBS Lett 596:1445-1452 (2022). PubMed: 35182436